MersV1, Main, Exploration, bibRecord, 004A25

Multi-pin peptide synthesis strategy for T cell determinant analysis

Identifieur interne : 004A25 ( Main/Exploration ); précédent : 004A24; suivant : 004A26

Multi-pin peptide synthesis strategy for T cell determinant analysis

Auteurs : N. Joe Maeji [Australie] ; Andrew M. Bray [Australie] ; H. Mario Geysen [Australie]

Source :

Journal of Immunological Methods [ 0022-1759 ] ; 1990.

RBID : ISTEX:EC4E0A94AABBFFC873B1069AC69DE614E9626120

English descriptors

Teeft :
- Acetic acid, Amino, Amino acid, Amino acid analyses, Amino acid analysis, Amino acids, Antibody binding, Assay, Cell clone, Cell culture assays, Cell determinant, Cell determinant analysis, Cell determinant studies, Cell determinants, Cell epitopes, Cell proliferation, Chem, Cleavable, Cleavable linker, Cleavable linker group, Cleavage, Cleavage procedure, Cleavage rate, Cleavage solution, Commonwealth serum laboratories, Cyanogen bromide, Ddalinstkiysyfpsvi skvnqgaqgi, Deprotection, Determinant, Diketopiperazine, Diketopiperazine formation, Epitope, Fmoc group, Geysen, Good purity, Hplc, Hplc traces, Hydroxide, Intramolecular aminolysis, Ionic strength, Large numbers, Linker, Linker groups, Many peptides, Microtitre plates, Minimum reactive sequence, Nmol, Organic solvents, Peptide, Peptide cleavage, Peptide solutions, Peptide synthesis, Phosphate buffer, Polyethylene pins, Room temperature, Side chain deprotection, Side reaction, Simultaneous synthesis, Sodium hydroxide, Solid phase peptide synthesis, Terminal acetylated, Terminal moiety, Tetanus toxin, Trifluoroacetic acid, Tritiated thymidine, Waters associates.

Abstract

Abstract: Techniques to synthesize many peptides simultaneously exist, however their individual cleavage and subsequent purification constitutes a bottleneck to total throughput. Biological screening of peptides is generally carried out at physiological pH in aqueous solutions. However, peptides, unless individually purified are usually contaminated by residual compounds used in their preparation such as trifluoroacetic acid, organic solvents, scavengers etc. In testing with cellular systems, such as T cell determinant analysis, such contaminations must be rigorously excluded. We have extended the pin synthesis technique of synthesizing and screening large number of peptides (Geysen et al., 1984) to the analysis of T cell determinants. Peptides can be synthesized on polyethylene pins, the side chain protective groups removed and the peptides washed free of contaminants. A linker system stable under these conditions can then be triggered to cleave the peptides from the pins in an aqueous solution at neutral pH. This strategy enables the rapid mapping of T cell determinants. It is also applicable to other systems where large numbers of solution phase peptides are required, for example, in the study of hormone analogues.

Url:

https://api.istex.fr/ark:/67375/6H6-JNQG5VDH-G/fulltext.pdf

DOI: 10.1016/0022-1759(90)90108-8

Affiliations:

Australie

Links toward previous steps (curation, corpus...)

to stream Istex, to step Corpus: 001F85
to stream Istex, to step Curation: 001F85
to stream Istex, to step Checkpoint: 001F50
to stream Main, to step Merge: 004B03
to stream Main, to step Curation: 004A25

Le document en format XML

<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Multi-pin peptide synthesis strategy for T cell determinant analysis</title>
<author><name sortKey="Joe Maeji, N" sort="Joe Maeji, N" uniqKey="Joe Maeji N" first="N." last="Joe Maeji">N. Joe Maeji</name>
</author>
<author><name sortKey="Bray, Andrew M" sort="Bray, Andrew M" uniqKey="Bray A" first="Andrew M." last="Bray">Andrew M. Bray</name>
</author>
<author><name sortKey="Mario Geysen, H" sort="Mario Geysen, H" uniqKey="Mario Geysen H" first="H." last="Mario Geysen">H. Mario Geysen</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:EC4E0A94AABBFFC873B1069AC69DE614E9626120</idno>
<date when="1990" year="1990">1990</date>
<idno type="doi">10.1016/0022-1759(90)90108-8</idno>
<idno type="url">https://api.istex.fr/ark:/67375/6H6-JNQG5VDH-G/fulltext.pdf</idno>
<idno type="wicri:Area/Istex/Corpus">001F85</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001F85</idno>
<idno type="wicri:Area/Istex/Curation">001F85</idno>
<idno type="wicri:Area/Istex/Checkpoint">001F50</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001F50</idno>
<idno type="wicri:doubleKey">0022-1759:1990:Joe Maeji N:multi:pin:peptide</idno>
<idno type="wicri:Area/Main/Merge">004B03</idno>
<idno type="wicri:Area/Main/Curation">004A25</idno>
<idno type="wicri:Area/Main/Exploration">004A25</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a">Multi-pin peptide synthesis strategy for T cell determinant analysis</title>
<author><name sortKey="Joe Maeji, N" sort="Joe Maeji, N" uniqKey="Joe Maeji N" first="N." last="Joe Maeji">N. Joe Maeji</name>
<affiliation wicri:level="1"><country xml:lang="fr">Australie</country>
<wicri:regionArea>Coselco Mimotopes Pty. Ltd. (a wholly owned subsidiary of the Commonwealth Serum Laboratories), Cnr Duerdin & Martin Streets, P.O. Box 40, Clayton, Victoria</wicri:regionArea>
<wicri:noRegion>Victoria</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Bray, Andrew M" sort="Bray, Andrew M" uniqKey="Bray A" first="Andrew M." last="Bray">Andrew M. Bray</name>
<affiliation wicri:level="1"><country xml:lang="fr">Australie</country>
<wicri:regionArea>Coselco Mimotopes Pty. Ltd. (a wholly owned subsidiary of the Commonwealth Serum Laboratories), Cnr Duerdin & Martin Streets, P.O. Box 40, Clayton, Victoria</wicri:regionArea>
<wicri:noRegion>Victoria</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Mario Geysen, H" sort="Mario Geysen, H" uniqKey="Mario Geysen H" first="H." last="Mario Geysen">H. Mario Geysen</name>
<affiliation wicri:level="1"><country xml:lang="fr">Australie</country>
<wicri:regionArea>Coselco Mimotopes Pty. Ltd. (a wholly owned subsidiary of the Commonwealth Serum Laboratories), Cnr Duerdin & Martin Streets, P.O. Box 40, Clayton, Victoria</wicri:regionArea>
<wicri:noRegion>Victoria</wicri:noRegion>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Journal of Immunological Methods</title>
<title level="j" type="abbrev">JIM</title>
<idno type="ISSN">0022-1759</idno>
<imprint><publisher>ELSEVIER</publisher>
<date type="published" when="1990">1990</date>
<biblScope unit="volume">134</biblScope>
<biblScope unit="issue">1</biblScope>
<biblScope unit="page" from="23">23</biblScope>
<biblScope unit="page" to="33">33</biblScope>
</imprint>
<idno type="ISSN">0022-1759</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0022-1759</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Acetic acid</term>
<term>Amino</term>
<term>Amino acid</term>
<term>Amino acid analyses</term>
<term>Amino acid analysis</term>
<term>Amino acids</term>
<term>Antibody binding</term>
<term>Assay</term>
<term>Cell clone</term>
<term>Cell culture assays</term>
<term>Cell determinant</term>
<term>Cell determinant analysis</term>
<term>Cell determinant studies</term>
<term>Cell determinants</term>
<term>Cell epitopes</term>
<term>Cell proliferation</term>
<term>Chem</term>
<term>Cleavable</term>
<term>Cleavable linker</term>
<term>Cleavable linker group</term>
<term>Cleavage</term>
<term>Cleavage procedure</term>
<term>Cleavage rate</term>
<term>Cleavage solution</term>
<term>Commonwealth serum laboratories</term>
<term>Cyanogen bromide</term>
<term>Ddalinstkiysyfpsvi skvnqgaqgi</term>
<term>Deprotection</term>
<term>Determinant</term>
<term>Diketopiperazine</term>
<term>Diketopiperazine formation</term>
<term>Epitope</term>
<term>Fmoc group</term>
<term>Geysen</term>
<term>Good purity</term>
<term>Hplc</term>
<term>Hplc traces</term>
<term>Hydroxide</term>
<term>Intramolecular aminolysis</term>
<term>Ionic strength</term>
<term>Large numbers</term>
<term>Linker</term>
<term>Linker groups</term>
<term>Many peptides</term>
<term>Microtitre plates</term>
<term>Minimum reactive sequence</term>
<term>Nmol</term>
<term>Organic solvents</term>
<term>Peptide</term>
<term>Peptide cleavage</term>
<term>Peptide solutions</term>
<term>Peptide synthesis</term>
<term>Phosphate buffer</term>
<term>Polyethylene pins</term>
<term>Room temperature</term>
<term>Side chain deprotection</term>
<term>Side reaction</term>
<term>Simultaneous synthesis</term>
<term>Sodium hydroxide</term>
<term>Solid phase peptide synthesis</term>
<term>Terminal acetylated</term>
<term>Terminal moiety</term>
<term>Tetanus toxin</term>
<term>Trifluoroacetic acid</term>
<term>Tritiated thymidine</term>
<term>Waters associates</term>
</keywords>
</textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Abstract: Techniques to synthesize many peptides simultaneously exist, however their individual cleavage and subsequent purification constitutes a bottleneck to total throughput. Biological screening of peptides is generally carried out at physiological pH in aqueous solutions. However, peptides, unless individually purified are usually contaminated by residual compounds used in their preparation such as trifluoroacetic acid, organic solvents, scavengers etc. In testing with cellular systems, such as T cell determinant analysis, such contaminations must be rigorously excluded. We have extended the pin synthesis technique of synthesizing and screening large number of peptides (Geysen et al., 1984) to the analysis of T cell determinants. Peptides can be synthesized on polyethylene pins, the side chain protective groups removed and the peptides washed free of contaminants. A linker system stable under these conditions can then be triggered to cleave the peptides from the pins in an aqueous solution at neutral pH. This strategy enables the rapid mapping of T cell determinants. It is also applicable to other systems where large numbers of solution phase peptides are required, for example, in the study of hormone analogues.</div>
</front>
</TEI>
<affiliations><list><country><li>Australie</li>
</country>
</list>
<tree><country name="Australie"><noRegion><name sortKey="Joe Maeji, N" sort="Joe Maeji, N" uniqKey="Joe Maeji N" first="N." last="Joe Maeji">N. Joe Maeji</name>
</noRegion>
<name sortKey="Bray, Andrew M" sort="Bray, Andrew M" uniqKey="Bray A" first="Andrew M." last="Bray">Andrew M. Bray</name>
<name sortKey="Mario Geysen, H" sort="Mario Geysen, H" uniqKey="Mario Geysen H" first="H." last="Mario Geysen">H. Mario Geysen</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 004A25 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 004A25 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    MersV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     ISTEX:EC4E0A94AABBFFC873B1069AC69DE614E9626120
   |texte=   Multi-pin peptide synthesis strategy for T cell determinant analysis
}}

This area was generated with Dilib version V0.6.33.
Data generation: Mon Apr 20 23:26:43 2020. Site generation: Sat Mar 27 09:06:09 2021

	Serveur d'exploration MERS
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration MERS

Multi-pin peptide synthesis strategy for T cell determinant analysis

Multi-pin peptide synthesis strategy for T cell determinant analysis

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri